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ctpB [2019-07-12 09:36:27]
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ctpB [2019-07-12 09:36:27]

carboxy-terminal processing serine protease, cleaves SpoIVFA, this results in processing of pro-SigK
Locus
BSU35240
Isoelectric point
8.64
Molecular weight
52.63 kDa
Protein length
480 aa Sequence Blast
Gene length
1443 bp Sequence Blast
Function
control of SigK activation
Product
carboxy-terminal processing serine protease, cleaves SpoIVFA
Essential
no
Synonyms
yvjB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Proteolysis → Additional proteins involved in proteolysis
  • Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    3,622,356 3,623,798

    The protein

    Protein family

  • Peptidase s41a family (together with CtpA) (according to Uniprot)
  • PDZ protease PubMed

    • Paralogous protein(s)

  • CtpA

    • Domains

  • signal peptide (aa 1 - 23) (according to Uniprot)
  • PDZ domain (aa 92 - 182) (according to Uniprot)
  • S41 peptidase domain (by similarity to CtpA) PubMed
  • peptidoglycan binding domain (C-terminal) (by similarity to CtpA) PubMed

    • Effectors of protein activity

  • substrate binding of CtpB induces opening of the PDZ gate and protease activation PubMed
  • CtpB acts in concert with a second signaling protease, SpoIVB PubMed

    • Structure

  • 4C2C PubMed
  • forms a ring-like scaffold with two narrow tunnels for substrate binding PubMed

    • Localization

  • intracellular space between the mother cell and the forespore PubMed

    • Expression and Regulation

    Operons

    Genes
    ctpB
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulation

  • expressed during sporulation (SigE, SigG) PubMed
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    Biological materials

    Mutant

  • MGNA-A074 (yvjB::erm), available at the NBRP B. subtilis, Japan
  • 1S129 ( ctpB::tet), PubMed, available at BGSC
  • BKE35240 (ctpB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT
  • BKK35240 (ctpB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT

    • References

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